Staff / Senior Scientist, Bioconjugation Chemistry

About Glyphic: At Glyphic Biotechnologies, we plan to create the protein revolution for which scientists and researchers have been waiting. We are developing a massively parallel, single-molecule proteome sequencing platform that will transform life science discovery and usher in a new era of insights into human biology and disease. To date, we have raised >$80M from venture partners and non-dilutive grant funding to achieve our vision of next generation proteome sequencing. What we are looking for in you We are seeking a Senior or Staff Scientist, Bioconjugation to join our Chemistry team at a pivotal stage in assay development. Reporting to the Senior Director of Chemistry, you will be a hands-on expert who designs, develops, and executes bioconjugation strategies for peptide and oligonucleotide components that are central to our single-molecule proteome sequencing assay. The ideal candidate brings deep expertise in protein and peptide bioconjugation, site-specific chemical modification of amino acid sidechains, and oligonucleotide-biomolecule conjugation, with a track record of working in complex, multi-component systems (e.g., ADCs, therapeutic bioconjugates, or new bioconjugated components for assays). A successful candidate will have an innovative method-development mindset and will effectively build new approaches to making bioconjugates. This individual will thrive in a cross-functional environment where chemical solutions must be tightly integrated into assay design. This role offers the unique opportunity to develop first-in-class conjugation chemistry that enables single-molecule protein sequencing.  This is a full-time, exempt, in-person position with work conducted at our Berkeley, CA location. What you’ll do Bioconjugation strategies: Partner with sample prep and assay development to screen and select conjugation chemistries for peptides and oligonucleotides that enable single molecule protein sequencing Design bioconjugation workflows using NHS-ester chemistry, maleimides, isothiocyanate chemistry, and click chemistry (CuAAC, SPAAC, IEDDA)  Prepare bioconjugation reagents to enable  studies from proof-of-concept through prototype-kitting. Peptide chemistry: Develop labeling strategies based on Edman Chemistry Develop and apply selective C-terminal and N-terminal conjugation chemistries Develop site-specific labeling strategies for amino acid sidechains (Cys, Lys, Arg, Tyr, Trp, His, Asp/Glu) on native peptides. Screen protecting groups to enhance amino acid signal in the protein sequencing assay Enable on-bead workflow to label and release peptides for interrogation in the sequencing assay Synthesize, characterize, and scale: Synthesize and purify peptide and oligonucleotide conjugates for assay testing Characterize conjugation efficiency, purity, and stability using tools such as HPLC, LC-MS/MS, NMR, CE, UV/Vis, and FTIR Scale conjugate synthesis from R&D quantities to prototype-grade supply for internal t...